中国普外基础与临床杂志

中国普外基础与临床杂志

长链非编码 RNA 母系表达基因 3 多态性与胃癌关系的研究

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目的 探讨长链非编码 RNA 母系表达基因 3(MEG3)多态性与胃癌的关系。 方法 选择 2013 年 3 月至 2017 年 10 月期间于云南省肿瘤医院住院治疗的汉族胃癌患者 172 例(胃癌组)及健康体检汉族个体 224 人(对照组)作为研究对象。采用 TaqMan 探针法对 MEG3 两个标签单核苷酸多态性(rs7158663 和 rs4081134)进行分型,并分析其与胃癌及其临床病理特征的关系。 结果 MEG3 rs7158663 以 GG 基因型作为参照,经 χ2 检验并校正年龄和性别后发现 AG+AA 基因型在胃癌组中的分布频率明显高于对照组[校正 OR=1.71,95%CI 为(1.14,2.56),P=0.010];以 G 等位基因作为参照,经 χ2 检验并校正年龄和性别后发现 A 等位基因在胃癌组中的分布频率明显高于对照组[校正 OR=1.58,95%CI(1.15,2.19),P=0.005]。rs4081134 多态性的基因型和等位基因在 2 组间比较差异均无统计学意义(P>0.017)。MEG3 rs7158663 和 rs4081134 两位点多态性与胃癌患者的临床病理特征均无关(P>0.017)。 结论 MEG3 rs7158663 AG+AA 基因型可能是中国汉族人群胃癌的易感基因之一。

Objective To explore relationship between long non-coding RNA maternally expressed gene 3 (MEG3) polymorphisms and risk of gastric cancer. Methods One hundred and seventy-two Han patients with gastric cancer (gastric cancer group) and 224 Han individuals for physical examination (control group) in the Yunnan Cancer Hospital from March 2013 to October 2017 were selected as subjects. The rs7158663 and rs4081134 polymorphisms of the MEG3 were genotyped by using a TaqMan technique. The associations between the 2 polymorphisms and the risk of the gastric cancer and its clinical features were analyzed using the SPSS software. Results The frequencies of the AG+AA genotype and the A allele of the MEG3 rs7158663 in the gastric cancer group were significantly higher than those in the control group using the GG genotype and G allele as a reference respectively [adjusted OR=1.71, 95%CI (1.14, 2.56), P=0.010; adjusted OR=1.58, 95%CI (1.15, 2.19), P=0.005] after the Chi-square test and the adjustment of age and gender. The frequencies of the AG+AA genotype and the A allele of the MEG3 rs4081134 had no significant differences between the gastric cancer group and the control group (P>0.017). Moreover, the polymorphisms of the MEG3 rs7158663 and rs4081134 were not associated with the clinical features of the gastric cancer (P>0.017). Conclusion MEG3 rs7158663 AG+AA genotype might be one of susceptibility gene of gastric cancer in Chinese Han population.

关键词: 长链非编码 RNA; 母系表达基因 3; 单核苷酸多态性; 胃癌

Key words: long non-coding RNA; maternally expressed gene 3; single nucleotide polymorphism; gastric cancer

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