中国普外基础与临床杂志

中国普外基础与临床杂志

长链非编码 RNA GAS5 在肝细胞性肝癌中的表达及其临床意义

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目的 探讨长链非编码 RNA 中的生长停滞敏感基因 5(GAS5)mRNA 在肝细胞性肝癌组织中的表达及其临床意义。 方法 回顾性收集 2013 年 9 月至 2016 年 7 月期间于重庆医科大学附属第一医院行根治性切除手术、术后病理学检查证实为原发性肝细胞性肝癌的 120 例患者的癌组织及其癌旁组织,应用实时荧光定量(RT-PCR)法检测 2 类组织中 GAS5 mRNA 的表达,并分析其表达与肝细胞性肝癌患者临床病理学特征的关系,同时探索其对肝细胞性肝癌患者预后的影响。 结果 肝细胞性肝癌组织中 GAS5 mRNA 的表达水平低于癌旁组织(P<0.001)。GAS5 mRNA 的表达与患者的与肿瘤直径、肿瘤数量、术前甲胎蛋白(AFP)水平、淋巴结转移、TNM 临床分期和肿瘤分化程度均相关(P<0.05)。Cox 比例风险回归模型结果表明,GAS5 mRNA 的表达与预后有关(P<0.05)。 结论 GAS5 mRNA 有望成为肝细胞性肝癌早期诊断和预后判断的标志物。

Objective To investigate the expression of growth arrest-specific 5 (GAS5) mRNA and its clinical significance in hepatocellular carcinoma. Methods The expression of GAS5 mRNA in the hepatocellular carcinoma tissue and corresponding adjacent tissues were detected by real time-PCR. The relationship between the expression of GAS5 mRNA and clinicopathological characteristics were analyzed by SPSS 17.0 software. Results The expression of GAS5 mRNA in hepatocellular carcinoma tissues was significantly lower than that in the adjacent tissues (P<0.001). The expression of GAS5 mRNA was positive related to tumor size, lymph node metastasis, microvascular invasion, clinical stage, alpha fetoprotein level, and tumor differentiation (P<0.05). Cox hazard model results showed that low expression of GAS5 mRNA was associated with poor prognosis (P<0.05). Conclusion GAS5 mRNA was expected to become a diagnostic and prognostic marker for patients with hepatocellular carcinoma.

关键词: 肝细胞性肝癌; GAS5; 长链非编码 RNA; 预后

Key words: hepatocellular carcinoma; growth arrest-specific 5; long non-coding RNA; prognosis

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