中国普外基础与临床杂志

中国普外基础与临床杂志

干扰素γ预防大鼠腹部手术后肠粘连的实验研究

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目的 观察干扰素 γ 预防大鼠腹部手术后肠粘连的效果。 方法 选用 Wistar 大鼠 60 只,雌雄各半,采用随机数字表法随机分为假手术组、模型组、地塞米松组及干扰素 γ 组,其中干扰素 γ 组再进一步分为低、中、高浓度 3 个亚组,每组 10 只大鼠。除假手术组外,其余各组均开腹行锉刀摩擦盲肠术建立肠粘连模型。假手术组及模型组腹腔注射 4 mL/kg 生理盐水,地塞米松组腹腔注射地塞米松 10 mg/kg,干扰素 γ 低、中及高浓度 3 个亚组分别腹腔注射浓度为 7.5×104、1.5×105及 3.0×105 U/kg 干扰素 γ 溶液。各组均于术后第 8 天开腹对大鼠盲肠粘连程度进行 Nair 等级分级,同时取相应的盲肠组织检测其羟脯氨酸含量并经 HE 染色在镜下观察组织病理学变化情况。 结果 ① 肠粘连情况:与假手术组比较,其余各组大鼠均发生肠粘连且肠粘连 Nair 分级评价粘连程度均明显严重(P<0.05);与模型组比较,地塞米松组和干扰素 γ 中、高浓度 2 个亚组的肠粘连程度均明显减轻(P<0.05),但干扰素 γ 低浓度亚组与模型组比较差异无统计学意义(P>0.05);与地塞米松组比较,干扰素 γ 高浓度亚组的粘连程度明显减轻(P<0.05),其抗粘连效应明显,干扰素 γ 低、中浓度 2 个亚组与地塞米松组比较其抗粘连效应无差异(P>0.05)。② 镜下观察组织病理学改变结果:与地塞米松组相比,高浓度干扰素 γ 能有效减少纤维组织的发生和炎性细胞的浸润,肠壁肌层结构完整,少许炎症细胞散在浸润。③ 羟脯氨酸含量:与假手术组相比,模型组、地塞米松组和干扰素 γ 各亚组的盲肠组织中羟脯氨酸水平均显著升高(P<0.05);与模型组比较,地塞米松组和干扰素 γ 中、高浓度 2 个亚组的盲肠组织中羟脯氨酸含量均明显降低(P<0.05),但干扰素 γ 低浓度亚组与模型组间比较差异无统计学意义(P>0.05);与地塞米松组比较,干扰素 γ 高浓度亚组的盲肠组织中羟脯氨酸含量明显降低(P<0.05),但干扰素 γ 低、中浓度 2 个亚组与地塞米松组比较差异无统计学意义(P>0.05)。 结论 大鼠腹部手术后腹腔注射干扰素 γ 能有效降低肠粘连的发生。

Objective To observe effect of interferon-γ in preventing intestinal adhesion following abdominal surgery in rats. Methods A total of 60 Wistar rats were selected, which were randomly divided into a sham operation (SO) group, model group, dexamethasone (DXMS) group, and interferon-γ group, then the interferon-γ group was randomly divided into a low, medium, and high concentrations subgroups. Except for the SO group, the laparotomies with file friction caecum were performed for all the other groups to establish the intestinal adhesion model. SO group and model group were intraperitoneally injected with saline for 4 mL/kg. The 10 mg/kg dexamethasone was injected into the abdominal cavity of rats in the DXMS group. The 7.5×104, 1.5×105, and 3.0×105 U/kg interferon-γ concentrations were injected into the abdominal cavity of rats in the low, medium, and high concentrations subgroups respectively. The Nair grading of cecum adhesion degree was assessed on the 8th day after the surgery, then the histopathological change was observed by the HE staining under the microscopy and the hydroxyproline content in the cecum tissue was detected. Results ① The intestinal adhesion: Compared with the SO group, the intestinal adhesions occurred in all the other groups and the degrees of intestinal adhesions evaluated by the Nair grading were more significantly serious (P<0.05), which in the DXMS group and the medium and high concentrations of interferon-γ subgroups were significantly reduced (P<0.05) as compared with the model group, which in the high concentration of interferon-γ subgroup was significantly reduced (P<0.05) as compared with the DXMS group. ② The microscopic observation of histopathological results: Compared with the DXMS group, the high concentration of interferon-γ could effectively reduce the occurrence of fibrous tissue and inflammatory cell infiltration, the intestinal wall muscular layer structure was complete, a few inflammatory cells scattered in the infiltration. ③ The hydroxyproline content: The contents of hydroxyproline in the cecum tissue of the model group, DXMS group, and interferon-γ subgroups were significantly increased (P<0.05) as compared with the SO group, which of the DXMS group and medium and high concentrations of interferon-γ subgroups were significantly decreased (P<0.05) as compared with the model group, which of the high concentration of interferon-γ subgroup was significantly decreased (P<0.05) as compared with the DXMS group. Conclusion Interferon-γ has a preventive and therapeutic effect on postoperative intestinal adhesion and cecum injuries.

关键词: 干扰素 γ; 成纤维细胞; 白细胞介素-13; 肠粘连

Key words: interferon γ; fibroblast; interleukin 13; intestinal adhesion

引用本文: 李声璧, 黎祥涛, 周金宏, 赵琦, 翁启芳. 干扰素γ预防大鼠腹部手术后肠粘连的实验研究. 中国普外基础与临床杂志, 2019, 26(1): 19-24. doi: 10.7507/1007-9424.201810011 复制

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